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How to Fill Out FDA Form 2253 (w/Examples) + FAQs

FDA Form 2253 is the official transmittal document required when pharmaceutical companies submit promotional materials for approved prescription drugs and biologics to the FDA. Title 21 CFR 314.81(b)(3)(i) mandates that drug applicants submit specimens of all advertising and promotional labeling at the time of initial dissemination or publication—failure to comply renders the product misbranded under Section 502(n) of the Federal Food, Drug, and Cosmetic Act.

In September 2025 alone, the FDA’s Office of Prescription Drug Promotion (OPDP) issued approximately 130 warning and untitled letters targeting misleading promotional materials—a historic enforcement surge that signals intensified scrutiny of drug advertising compliance.

Here’s what you’ll learn in this guide:

  • 📋 Exactly how to complete each field on Form 2253, with real examples and material type codes
  • ⚖️ The legal requirements behind promotional submissions and the consequences of non-compliance
  • 💻 Whether to submit electronically via eCTD or through paper—and the mandatory deadlines you cannot miss
  • ⚠️ The most common submission errors that trigger FDA rejections and how to avoid them
  • ✅ Do’s and Don’ts from regulatory experts to ensure your promotional materials pass FDA review

What Is FDA Form 2253 and Who Must File It?

FDA Form 2253—formally titled “Transmittal of Advertisements and Promotional Labeling for Drugs and Biologics for Human Use”—serves as the cover sheet that accompanies every promotional material submission to the FDA. The form identifies the drug product, lists each promotional piece being submitted, and provides contact information for the responsible regulatory official.

Products Requiring Form 2253 Submissions

Application TypeProducts CoveredSubmitting Office
NDA (New Drug Application)Brand-name prescription drugsOPDP (CDER)
ANDA (Abbreviated New Drug Application)Generic prescription drugsOPDP (CDER)
BLA (Biologics License Application)CDER-regulated biologics (therapeutic proteins, monoclonal antibodies)OPDP (CDER)
BLA (CBER-regulated)Vaccines, blood products, gene therapiesAPLB (CBER)
CDER IND (Emergency Use Authorization)Products with authorized EUAsOPDP (CDER)

The submission requirement applies to the applicant (the holder of the NDA, ANDA, or BLA). If the manufacturer and distributor are different companies, either may submit the materials—but the applicant remains ultimately responsible for compliance under 21 CFR 314.81(b)(3).

What Qualifies as “Promotional Material”?

Promotional labeling encompasses any labeling other than FDA-required labeling that is devised to promote a drug product. This includes brochures, sales aids, direct mail pieces, websites, social media content, television commercials, radio ads, and even scripts for telephone calls. The FDA distinguishes between:

  • Advertising: Communications disseminated through broadcast (TV, radio) or print media directed at consumers or healthcare professionals
  • Promotional Labeling: Written, printed, audio, or visual matter distributed by or on behalf of the manufacturer that accompanies or references the drug

Both categories require Form 2253 submission at the time of initial use.


The Regulatory Framework: Why Form 2253 Exists

The legal foundation for Form 2253 submissions comes from 21 CFR 314.81(b)(3)(i), which states that applicants “shall submit specimens of mailing pieces and any other labeling or advertising devised for promotion of the drug product at the time of initial dissemination of the labeling and at the time of initial publication of the advertisement.”

Consequences of Non-Compliance

ViolationConsequence
Failure to submit promotional materialsProduct misbranding under FDCA Section 502(n)
Misleading or unbalanced promotional claimsWarning letter, untitled letter, or cease-and-desist order
False promotional materials for accelerated approval productsExpedited withdrawal of product approval under Section 506(c)
Repeated violationsCivil monetary penalties, injunctions, or criminal prosecution

A September 2025 warning letter to pharmaceutical manufacturers stated: “FDA requests [Company] take immediate action to address any violations (including, for example, ceasing and desisting promotional communications that are misleading).” This language reflects the FDA’s heightened enforcement posture.


Step-by-Step Instructions: How to Complete Each Field

The FDA provides official instructions for completing Form 2253. Below is a detailed breakdown of each field with practical guidance.

Box 1: Date Submitted

Enter the date you are sending the form and materials to the FDA. Use the drop-down calendar or MM/DD/YYYY format. This date establishes when you fulfilled your regulatory obligation. For promotional pieces that will be disseminated on a specific date, ensure the submission date is on or before that dissemination date.

Box 2: Application Information

Select the application type from the drop-down menu:

  • NDA (New Drug Application)
  • ANDA (Abbreviated New Drug Application)
  • BLA (Biologics License Application)
  • PMA (Premarket Approval Application)
  • CDER IND (for authorized Emergency Use Authorization products only)

Enter the six-digit application number. For CBER BLAs, include the supplement number if known.

Single vs. Multiple Products:

ScenarioSelectionRequirements
Promotional piece for one drugSingle ProductOne Form 2253 per application number
Price list mentioning multiple drugsMultiple ProductsList lead application on form; attach sheet with other application numbers, trade names, and established names
Corporate communicationsMultiple ProductsInclude labeling for each referenced product

Example: A formulary kit containing information about three different drugs from the same manufacturer would require checking “Multiple Products” and attaching a supplementary sheet listing all three application numbers.

Box 3: Proprietary Name

Enter the brand name of the drug product. Include the dosage form if it distinguishes the product from other forms with the same trade name.

Example: “Lipitor Tablets” or “Humira Injection”

Box 4: Established Name

Provide the generic (established) name of the drug. For biological products, include the Product Code Number if known or used.

Example: “atorvastatin calcium” or “adalimumab”

Box 5: Package Insert Date and ID Number

Enter the date and identification number of the most current FDA-approved product labeling. This is critical because promotional materials must be consistent with approved labeling. For paper submissions, include two copies of the package insert.

Box 6: Manufacturer Name and License Number

Provide the manufacturer’s name. For biological product submissions, also include the biologics license number.

Box 7: Advertising/Promotional Labeling Materials

This section requires detailed listing of each promotional piece. Add a new row for each item. Individual components of kits should be listed separately.

Box 7a: Professional or Consumer

Select the primary intended audience. If materials target both audiences, select based on the primary audience and note in Comments (Box 7f) that the piece will be viewed by both.

Box 7b: Material Type Codes

The FDA uses specific codes for material types. Use the exact FDA terminology:

FDA CodeDescriptionExample
Print AdPrint AdvertisementJournal ad in JAMA
TVTelevision Advertisement60-second DTC commercial
RadioAudio broadcast over radio waves30-second radio spot
Sales AidPrint detail aid for sales repsLeave-behind brochure for physicians
Electronic Detail AidElectronic apps/tools for sales repsiPad sales presentation
www-websiteInternet WebsiteProduct.com branded website
www-soc-medInternet Social MediaFacebook product page, Twitter posts
www-videoInternet VideoYouTube promotional video
www-mobileMobile TechnologySmartphone app, QR codes
www-bannerInternet BannerDisplay ads on medical websites
www-ecommInternet Electronic CommunicationPromotional emails
Direct MailPrinted non-electronic materials mailed to individualsPostcards to physicians
ReprintReproduction of previously printed materialJournal article reprint
Promotional LabelingGeneral category for brochures, booklets, price listsPatient brochure
Drug SampleSmall quantity given to prescribersSample starter packs
Training MaterialsLearning modules for HCPs or patientsInjection training video

Box 7c: Dissemination/Publication Date

Enter the date of initial dissemination or publication. This is the date the promotional piece first reaches its audience. Use the drop-down calendar or MM/DD/YYYY format.

Box 7d: Material ID Code

Provide your company’s internal identification code for the promotional piece. This helps track materials if the FDA has questions.

Box 7e: Material Description

Write your description of the promotional piece.

Example: “2-page journal advertisement for Healthcare Professional audience promoting efficacy in Type 2 Diabetes”

Box 7f: Comments

Include pertinent information about dissemination methods. Note if a piece is meant to be distributed exclusively with other pieces and identify the accompanying materials.

Boxes 8-12: Contact Information and Certification

  • Box 8: Applicant or agent’s return address including country
  • Box 9a-c: Telephone, fax, and email of the responsible official
  • Box 10: Typed name and title of the responsible official
  • Box 11: Signature of responsible official
  • Box 12: Date of signature

Box 13: For CBER Products Only

This box applies only to biologics regulated by CBER. Check “Draft” for pre-submission of draft materials or “Final” for postmarketing submissions of final materials. For OPDP submissions (CDER products), do not check either box.


Electronic vs. Paper Submission: Which Method to Use

Since June 24, 2021, the FDA mandates electronic submission via eCTD format for all required Form 2253 postmarketing submissions and accelerated approval pre-submissions. Paper submissions are no longer accepted for these mandatory categories.

Mandatory eCTD Submissions

Submission TypeRequired FormatSubmission Gateway
Form FDA 2253 (postmarketing)eCTD via ESGElectronic Submissions Gateway
Accelerated Approval Pre-submissionseCTD via ESGElectronic Submissions Gateway​

Optional Submission Formats

For non-mandatory submission types (voluntary advisory requests, general correspondence), the FDA accepts:

  • eCTD format via ESG
  • Non-eCTD electronic format via CDER NextGen Portal
  • Paper format via mail or delivery services

eCTD File Placement Requirements

DocumenteCTD Section
Form FDA 2253Section 1.1
Current Product LabelingSection 1.14.6
Promotional Materials (clean versions)Section 1.15.2.1.1
Annotated Materials (optional)Section 1.15.2.1.2

The FDA strongly recommends submitting a test submission to validate your format and ensure systems can receive your files before the actual submission.

Where to Send Submissions

Product TypeOfficeAddress/Method
CDER products (drugs, therapeutic biologics)OPDPElectronic via ESG; Paper to: FDA CDER OPDP, 5901-B Ammendale Road, Beltsville, MD 20705
CBER products (vaccines, blood products)APLBFDA CBER Document Control Center, 10903 New Hampshire Avenue, Building 71, Room G112, Silver Spring, MD 20993-0002

Timing Requirements: When to Submit

The timing of your submission depends on whether your product received standard or accelerated approval.

Standard Approval Products

Submission TypeTiming Requirement
Initial dissemination/publicationAt the time of initial use
Revised materialsAt the time of initial use of revised version

“At the time” means the submission should reach the FDA on or before the date the promotional piece is first disseminated or published.

Accelerated Approval Products (Subpart H and E)

Products approved under accelerated approval face heightened pre-submission requirements under 21 CFR 314.550 and 21 CFR 601.45.​

PeriodTiming Requirement
Launch Period (Days 1-120)Submit before approval date (during pre-approval review)​
Non-Launch Period (Day 121+)Submit at least 30 days prior to intended use​​
Final FormSubmit again on Form 2253 at time of actual use​

Example: If you want to use a promotional piece on Day 150 after approval, you must pre-submit it by Day 120 (30 days before use), then submit it again in final form on Form 2253 when you actually disseminate it.​


Real-World Scenarios: Action and Consequence Tables

Scenario 1: Launching a New DTC Television Campaign

A pharmaceutical company prepares a 60-second TV commercial for a newly approved diabetes medication.

ActionConsequence
Submit Form 2253 with TV ad storyboard, script, and final video on the day of first broadcastCompliant with 21 CFR 314.81(b)(3)(i); no enforcement action
Broadcast TV ad without submitting Form 2253Product misbranding; potential untitled or warning letter
Submit Form 2253 one week after broadcast beginsLate submission; FDA may note deficiency in compliance record
TV ad omits major statement about serious cardiovascular risksViolation of fair balance requirement; cease-and-desist letter likely

Scenario 2: Social Media Campaign for an Accelerated Approval Product

A biotech company wants to launch a Twitter campaign for an oncology drug approved under accelerated approval.

ActionConsequence
Pre-submit social media content 30 days before launch; submit final on Form 2253 at launchFull compliance with Subpart H requirements​
Post Twitter content without pre-submissionViolation of 21 CFR 314.550; grounds for expedited withdrawal
Social media posts claim drug “cures” cancer without adequate evidenceFalse promotional materials; expedited withdrawal proceedings possible
Include risk information via link to prescribing information (adequate provision)Potentially compliant if using appropriate FDA-recognized adequate provision methods

Scenario 3: Multiple Product Price List Submission

A manufacturer distributes a formulary price list mentioning five different prescription drugs.

ActionConsequence
Check “Multiple Products” on Form 2253; attach sheet listing all five application numbers with trade and established names; include labeling for each productCompliant submission
Check “Single Product” and list only one application numberForm error; FDA may reject or request amendment
Omit package inserts for three of the five productsValidation error 1551; resubmission required

Common Mistakes That Trigger FDA Rejections

The FDA’s Common Errors presentation identifies frequent submission problems. Avoiding these errors saves time and prevents compliance gaps.

General Submission Errors

MistakeWhy It’s a ProblemHow to Avoid
Incorrect Application NumberMaterials filed to wrong product recordDouble-check NDA/ANDA/BLA number before submission
Same Form 2253 for multiple application typesOnly one application type (NDA or BLA, not both) allowed per submissionCreate separate submissions for different application types
Audience type mismatchUS-regional file audience doesn’t match Form or cover letterVerify Professional vs. Consumer selection is consistent throughout
Issue date formatting errorMust be YYYYMMDD format in US-regional fileUse correct date format
Orphan files includedExtra files not referenced in submission structureRemove unused files before submission

Form 2253-Specific Errors

MistakeWhy It’s a ProblemHow to Avoid
Product labeling not in Section 1.14.6Triggers validation error 1551Always place current PI in Section 1.14.6
Materials not in Section 1.15.2.1Promotional materials in wrong eCTD locationFollow eCTD structure precisely
Including Form 356(h) with 2253 submissionOnly Form 2253 allowedRemove non-2253 forms
“Single Product” checked for grouped submissionMismatch with actual submission contentCheck “Multiple Products” for grouped submissions
Missing PI for all products in groupEach referenced product needs its labelingInclude labeling for every application in group

Mistakes to Avoid: Promotional Content

Beyond form errors, the FDA scrutinizes the promotional materials themselves. The September 2025 enforcement wave revealed key enforcement priorities.

MistakeNegative Outcome
Omitting or minimizing risk informationWarning letter; ads deemed misleading under FDCA Section 502(a)
Fast-paced visuals that distract from risk statementsFDA finds risk information incomprehensible; cease-and-desist
Claiming product is “FDA-approved” when it’s compoundedFalse claim; products misbranded under Section 502(a) and 502(bb)
Using “drug of choice” without superiority evidenceUnsubstantiated claim requiring supporting data
Reminder ads for drugs with boxed warningsReminder ads prohibited for such products; misbranding violation
Off-label promotionProduct misbranding; potential civil or criminal penalties
Using “new” beyond six months after launch“New” considered inaccurate after six months of marketing

Do’s and Don’ts for Form 2253 Submissions

Do’s

DoWhy
Use the most current version of Form FDA 2253Older versions may lack required fields; FDA rejects outdated forms
Submit a test eCTD submission before your first real filingValidates your format and confirms FDA systems can process your submission
Include annotated versions of promotional materialsWhile optional, annotations help FDA reviewers verify claims against labeling
List each kit component separately on Form 2253FDA requires individual listing for tracking purposes
Note mixed audiences in Comments fieldPrevents confusion if materials target both HCPs and consumers

Don’ts

Don’tWhy
Don’t combine different application types (NDA and BLA) in one submissionCauses validation failure; requires separate submissions
Don’t submit promotional materials as Reference Documents if omitted from prior submissionOmitted materials must go as Amendments, not Reference Documents
Don’t include Form 2253 for voluntary advisory requests to OPDPAdvisory submissions should not include Form 2253
Don’t check Draft/Final boxes for CDER productsBox 13 is only for CBER products
Don’t assume paper submission is still acceptableMandatory submissions require eCTD as of June 2021

Pros and Cons of Electronic (eCTD) Submission

Pros

BenefitExplanation
Faster processingElectronic submissions route directly to review offices; no manual handling delays​
Validation catches errors earlyeCTD validation tools identify format errors before FDA receipt
No mailing costs or delaysInstantaneous delivery via ESG
Better trackingReceive electronic acknowledgments confirming receipt
Required for complianceMandatory for postmarketing 2253 submissions since June 2021

Cons

DrawbackExplanation
Initial setup complexityRequires ESG account registration, publishing software, and learning eCTD specifications
Some materials difficult to represent electronicallyPhysical items (lenticular magnets, 3D pieces) require static images plus annotations or courtesy copies
Validation errors can delay submissionTechnical formatting issues may require resubmission
Software costseCTD publishing tools require investment
Test submission recommendedFDA recommends test submissions to validate format before going live

Key Organizations and Their Roles

Understanding which FDA office reviews your materials prevents misdirected submissions.

OrganizationRoleProducts Covered
OPDP (Office of Prescription Drug Promotion)Reviews promotional materials for prescription drugs; issues warning/untitled letters; provides advisory commentsNDAs, ANDAs, CDER-regulated BLAs
APLB (Advertising and Promotional Labeling Branch)Reviews promotional materials for CBER-regulated biologicsVaccines, blood products, gene therapies, CBER BLAs
ESG (Electronic Submissions Gateway)Secure portal for transmitting electronic regulatory submissionsAll eCTD submissions to CDER and CBER
CDER NextGen PortalAlternative electronic submission portal for non-eCTD submissions​Non-mandatory submissions only

FAQs

Does the FDA approve promotional materials before use?
No. Pre-approval is not required except in rare compliance actions or for accelerated approval products, which require pre-submission but not pre-approval.

Can reminder ads skip the brief summary requirement?
Yes. Reminder ads that mention only the drug name without indications or dosage recommendations are exempt from brief summary requirements under 21 CFR 202.1(e)(2)(i).

Are reminder ads allowed for drugs with boxed warnings?
No. Reminder advertisements are prohibited for prescription drugs whose labeling contains a boxed warning relating to a serious hazard.

Do generic drug manufacturers need to submit Form 2253?
Yes. ANDA holders must submit promotional materials at the time of initial dissemination just like NDA holders.

Can I submit Form 2253 by email?
No. Email submission is not accepted. Use eCTD via ESG for mandatory submissions or CDER NextGen Portal/mail for non-mandatory submissions.

How long can I use the word “new” in promotional materials?
Six months. OPDP considers “new” accurate for six months from initial marketing, not from FDA approval date.

Must foreign-language package inserts be submitted in English too?
Yes. Package inserts in other languages must also be submitted in English.

Who submits Form 2253 if manufacturer and distributor differ?
Either may submit, but the applicant (NDA/ANDA/BLA holder) remains ultimately responsible for compliance.

What is “fair balance” in promotional materials?
Fair balance requires that risk information be presented with prominence and readability reasonably comparable to benefit information.

Can I bundle multiple interactive materials in one submission?
Yes. The FDA allows bundling of interactive materials to reduce administrative burden for digital promotions.

What happens if my eCTD submission fails validation?
Resubmission required. Correct the error identified by the validation tool and resubmit with the corrected file.

Are OTC drug promotions subject to Form 2253?
No. Form 2253 applies to prescription drugs and biologics. OTC advertising is regulated by the FTC, though OTC labeling remains under FDA jurisdiction.